类器官与干细胞研究显微镜方案：球体成像、切片观察与长时程监测

500 µm 或需整体视野时，先评估透明化，再用光片做整体扫描。物镜比机身更影响成败——高 NA 油镜工作距离短、折射率与样本不匹配，进入厚样本会明显掉对比度[3]。"}}},"comments":[],"parent_id":"BfF1dBTDJozWtSxzOwkcdu8MnRc","revisions":[],"hidden":false,"align":"left","type":"text"}},"doxcnO2pQgEK5Ur92w8KMvV0hCe":{"id":"doxcnO2pQgEK5Ur92w8KMvV0hCe","snapshot":{"comments":[],"revisions":[],"hidden":false,"ai_extra":{"gen_sources":[2]},"text":{"apool":{"numToAttrib":{"0":["author","7592327302017452999"],"1":["ai-extra","{\"is_ai_gen\":true,\"gen_source\":2}"]},"nextNum":2,"attribToNum":{"author,7592327302017452999":0,"ai-extra,{\"is_ai_gen\":true,\"gen_source\":2}":1}},"initialAttributedTexts":{"text":{"0":"三、切片观察：形态学判读仍是基准"},"attribs":{"0":"*0*1+g"}}},"align":"left","type":"heading2","parent_id":"BfF1dBTDJozWtSxzOwkcdu8MnRc","locked":false,"author":"7592327302017452999","children":[]}},"doxcn6puypRxT42B91GNEbvpo6z":{"id":"doxcn6puypRxT42B91GNEbvpo6z","snapshot":{"type":"text","revisions":[],"locked":false,"author":"7592327302017452999","ai_extra":{"gen_sources":[2]},"parent_id":"BfF1dBTDJozWtSxzOwkcdu8MnRc","comments":[],"hidden":false,"children":[],"text":{"apool":{"numToAttrib":{"0":["author","7592327302017452999"],"1":["ai-extra","{\"is_ai_gen\":true,\"gen_source\":2}"]},"nextNum":2,"attribToNum":{"author,7592327302017452999":0,"ai-extra,{\"is_ai_gen\":true,\"gen_source\":2}":1}},"initialAttributedTexts":{"attribs":{"0":"*0*1+1p"},"text":{"0":"切片观察不能省：球体的分层、极性、腔隙形成与细胞类型分布，最终仍要靠切片形态学确认；镜下判断与切片互为依据，而不是二选一。"}}},"align":"left"}},"doxcnrGZkFWA3sF7iDvn4cbDTNQ":{"id":"doxcnrGZkFWA3sF7iDvn4cbDTNQ","snapshot":{"text":{"apool":{"numToAttrib":{"0":["author","7592327302017452999"],"1":["ai-extra","{\"is_ai_gen\":true,\"gen_source\":2}"]},"nextNum":2,"attribToNum":{"author,7592327302017452999":0,"ai-extra,{\"is_ai_gen\":true,\"gen_source\":2}":1}},"initialAttributedTexts":{"attribs":{"0":"*0*1+3d"},"text":{"0":"切片环节通常用明场/荧光正置显微镜配合 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